Quality of groundwater is considered to be of vital importance for all human beings and animals due to its extensive use for drinking purpose. In this connection, 60 water samples collected from rural areas of district DG Khan and Muzaffargarh, Pakistan were evaluated for physicochemical parameters (color, taste, odor, turbidity, pH and TDS), essential metals (Na+, K+, Ca+2, Mg+2), heavy metals (Mn, As, Fe, Zn, Cu, Ni, Cr) and microbiological organisms (total coliform and faecal coliform) which revealed that 47, 30, 27, 31, 57, 12, 8, 23 and 18% water samples were having high concentration of Ca, Na, K, Fe, As, Cu, TDS, total coliform and faecal coliform respectively. Calculation of sodium adsorption ratio (SAR) showed that 22% water samples were not suitable for irrigation purpose. Other than this, health risk assessment due to high content of heavy metals was carried out by calculating CDI and HRI for both adults and children separately. Calculated mean CDI and HRI were found in the order of As>Mn>Cr>Ni>Cu>Zn>Fe and As>Mn>Ni>Cu>Cr>Zn>Fe respectively and overall arsenic HRI>1 in 73 and 50% water samples for children and adults respectively. Pollution index (Pi) for Fe and As was significant among all heavy metals analyzed.