Objective: Human papillomavirus infection and the disease burden of cervical cancer are alarmingly increasing in the sub-Saharan region. Educational intervention is a primary preventive method that is effective in low-income countries like Ethiopia. This research aimed to determine the impact of knowledge-based intervention and factors influencing women\'s knowledge levels attending college education at the University of Gondar, Ethiopia. \nMethods: A cross-sectional comparative study was conducted from January to April 2018, and to assess the pre- and post-knowledge intervention, a simple random sampling method was used to select 283 study participants, and data were analyzed using SPSS ver23. \nResults: Two hundred eighty-three female study participants had a 100% response rate, and the mean age was 20.87 years (±SD, 1.838). There was an increase in overall awareness about cervical cancer (symptoms, risk factors, screening methods, and vaccination) in all post-intervened students compared to baseline knowledge levels (before educational intervention) statistically at p≤0.001 significance level. Initial awareness on various broad issues was 7.99, and after educational intervention, it was 26.84, with a mean overall knowledge increase of 18.85. Before the intervention, biological students were more aware than others [AOR: 4.6, 95%CI:(1.3-16.5], mothers with higher education levels were more knowledgeable over illiterate [AOR: 4.05, 95% CI:(1.14-14.29], family income ≤2000 ETB were less informed over the higher family income [AOR: 7.58, 95%CI:(1.64-34.94], significant on overall baseline knowledge levels, and after educational intervention, study participants third year graduation and above had overall awareness over the second year, and less [AOR: 0.29, 95%CI:(0.115-0.777] and study participants fathers with illiterate education level were more than two times [AOR: 2.88, 95%CI:(1.22-6.77] less improved awareness over 10th and above were the independent variables significant for the overall increase in knowledge levels. \nConclusion: The present study suggests that educational intervention as the primary preventive method is effective and increases positive attitudes to reduce cervical cancer burden.